Ibihe Byakurikiyeho-Ikoranabuhanga ryihutisha Imbeba Icyitegererezo
TurboMice ™ Ikoranabuhanga
Byatunganijwe na MingCeler binyuze murukurikirane rwo gutezimbere tetraploid yuzuzanya.
MuguhuzaIkorana buhanga rya Genen'imbeba nzizaEmbryonic Stem Cell Gutegura tekinoroji, ubu dushobora guhindura hafi intego zose za gen.
TurboMice ™ Ikoranabuhanga
Ikoranabuhanga ryacu ryiza cyane rya Tetraploid ryadushoboje kongera cyane kubyara imbeba kuva kuri 1% -5% kugeza kuri 30% -60% hamwe ningirabuzimafatizo zikomoka kuri gene, hafi yo guhuza imikorere yo kwimura urusoro rusanzwe.
MingCeler nisosiyete ya mbere kwisi yashohoje neza ihinduka rya tekinoroji ya Tetraploid kuva muri laboratoire ikajya mubikorwa byinganda.
Ikoranabuhanga gakondo
Ubuhanga gakondo bwo kwerekana inyamanswa, nka microinjection ya kirimbuzi hamwe na ES yibasiwe, bikubiyemo korora byibuze ibisekuruza 2 kugeza kuri 3 kugirango ubone imbeba za genotype ya homosexique, ubusanzwe ifata amezi 6-8.
Iyi nzira ndende ibangamira cyane iterambere niterambere ryiterambere ryibiyobyabwenge nubushakashatsi bwindwara.
Byihuse
1. imbeba zigera kuri 20 zidahuje igitsina zahinduwe imbereAmezi 2-4.
2. ≈ Imbeba 50 zidahuje igitsina muri ibi bikurikiraAmezi 2.
3. 400+ imbeba zidahuje igitsina imbereAmezi 8-12.
Raporo y'imanza muri 2020
TurboMice ™ tekinoroji yakoreshejwe kugirango itange umusaruro byihuse500 bahuje igitsinaimbeba ya ACE2 yimbeba imbere gusaAmezi 8kubushakashatsi bwa COVID-19 nibiyobyabwenge.
Guhitamo byoroshye
Ubuhanga gakondo bufite aho bugarukira muguhitamo imbeba, mugihe TurboMice ™ tekinoroji itanga uburyo bworoshye hamwe nubwoko butandukanye bwororoka kandi bwakorewe guhitamo (harimo Balb / c, ICR, C57BL / 6, nibindi).
Imbeba zo mu bwoko bwa F0 zubatswe ukoresheje TurboMice ™ tekinoroji ni imbeba zigenewe inkomoko imwe.
● Kubwibyo, ibikoresho bya genetike yimbeba F0 birasa, bigabanya amakosa yubushakashatsi yatewe nubwoko butandukanye.
● Ergo, amakuru yubushakashatsi bwo gusuzuma imikorere yibiyobyabwenge nubundi bushakashatsi burahuye kandi bwizewe.
Igumana ubunyangamugayo bwiza
Muri Situ Precision Gene Guhindura
● Muburyo bwiza bwo guhindura gene hamwe nurwego rwerekana imiterere ya gen.
Imbeba Yumuntu ya ACE2 yatejwe imbere nubuhanga gakondo itangwa no kumenyekanisha kwimikorere ya K18-ACE2 ya porotokoro, idashobora kugera ku guhindurwa neza bitewe no kwinjizamo ibintu ku buryo butemewe, bikavamo kubura umwirondoro w’imiterere ya ACE2 mu buryo bwimiterere y’imbeba ya ACE2 yubatswe. .
Imbeba ya MingCeler ya kimuntu ya ACE2 yerekana imvugo yihariye mubice bitandukanye (ibishushanyo C na D hejuru), kandi bigereranya neza ibiranga ivuriro nyuma yo kwandura SARS-CoV-2.
Iterambere ryihariye rya platform
●Porogaramu yihariye ya MingCeler EnhancerPlus irashobora gufasha abakiriya bacu kunoza urwego rwimiterere ya gen.
● Igisubizo cyimyaka myinshi yo gukusanya amakuru muri epigenetics hamwe nubushakashatsi bugena ibyateganijwe, urubuga rwa EnhancerPlus rwa MingCeler rushobora guhanura neza imyanya ndangagitsina ya Enhancers, bigatuma igishushanyo mbonera cyo guhindura gene gishingiye ku bimenyetso.
● Nkigisubizo, genes igenewe irashobora gusa neza nuburyo bwo kwerekana imiterere ninzego za endogenous.
Raporo y'ibyabaye: Ubumuntu bwa X gen.
Gukoresha urubuga rwa EnhancerPlus, ingamba zarakozwe neza kandi urwego rwerekana imvugo rwiyongereyeho ibintu bitatu byerekana ubunini (reba hejuru yubushushanyo), bigera kubisabwa kugirango iterambere ryibiyobyabwenge nkuko byagenwe nabakiriya kurwego rwa poroteyine.
● Nyuma yo kuzuza imbaga ya mbere ku isi imbeba za kimuntu za ACE2 mu 2020, MingCeler yazamuye imbeba y’imbeba ya ACE2 ikoresheje uburyo bune bwifashishijwe na EnhancerPlus, hamwe n’urwego rwo kwerekana imiterere ya ACE2 ya kimuntu amaherezo igera hafi y’imvugo ya imbeba ACE2.
Urwego rwo kwerekana imiterere ya ACE2 yumuntu mubice bitandukanye byimiterere yimbeba yabantu
Guhindura Gene-Guhindura Gene
● TurboMice ™ ikoranabuhanga niryo hitamo ryiza kumasoko yo kubyara imiterere-yimbeba ya gene-yahinduwe yimbeba yimbeba, itanga ibyiza byukuri muburyo bwo kwinjiza ahantu, kwinjiza ibice birebire, hamwe na loci nyinshi zahinduwe, nta kibazo cyo gutandukanya allelic.
● TurboMice ™ ikoranabuhanga ryemerera icyarimwe icyarimwe guhindura genes zigera kuri 3 kugirango habeho imiterere yimbeba ya homosexous mult-locus gene yimbeba yimbeba iturutse kumasemburo ya genoside ya embryonic stem selile, bitabaye ngombwa ko habaho uburyo bwo korora / gusuzuma igihe, bitanga ibintu bitandukanye ya moderi ifite agaciro gakomeye isabwa mubushakashatsi bushya bwibiyobyabwenge.
Ing MingCeler yateje imbere imbeba zitandukanye za loci zahinduwe na gene mugihe gito, gishingiye ku mbeba za ACE2 zabantu.
Igice kirekire
TurboMice ™ ikorana buhanga ituma gene ihindura neza ibice birebire birenga 20kb, byorohereza umusaruro wihuse wubwoko bugoye nko kuba umuntu, gukomanga ibintu (CKO), hamwe no gukomanga (KI).
Ibisohokayandikiro
[1] Wang G, Yang ML, Duan ZL, Liu FL, Jin L, Long CB, Zhang M, Tang XP, Xu L, Li YC, Kamau PM, Yang L, Liu HQ, Xu JW, Chen JK, Zheng YT , Peng XZ, Lai R. Dalbavancin ihuza ACE2 kugirango ihagarike imikoranire yayo na poroteyine ya SARS-CoV-2 kandi ifite akamaro mukurinda kwandura SARS-CoV-2 mubyitegererezo by'inyamaswa.Akagari ka Res.2021 Mutarama; 31 (1): 17-24.doi: 10.1038 / s41422-020-00450-0.(NIBA: 20.507)
[2] Liu FL, Wu K, Sun J, Duan Z, Quan X, Kuang J, Chu S, Pang W, Gao H, Xu L, Li YC, Zhang HL, Wang XH, Luo RH, Feng XL, Schöler HR , Chen X, Pei D, Wu G, Zheng YT, Chen J. Igisekuru cyihuse cya ACE2 cyimiterere yimbeba yimbeba ya COVID-19 hamwe na tetraploid yuzuzanya.Natl Sci Ibyah. 2020 Ugushyingo 24; 8 (2): nwaa285.doi: 10.1093 / nsr / nwaa285.(NIBA: 16.693)
Urujya n'uruza rwa serivisi
Icyo ukeneye gukora nukuduha ibisabwa kugirango uhindure gene ya moderi yimbeba yawe.Ukurikije ibisobanuro byawe, tuzashyira hamwe gahunda ibanza yihariye, hanyuma nyuma y'ibindi biganiro no kumvikana, tuzasinya amasezerano ya serivisi tekinike.Nyuma yo gutangira umushinga tuzatanga amakuru mugihe cyiterambere.