Imirongo yahinduwe
Imirongo ngengabuzima yahinduwe yerekeza ku ngirabuzimafatizo zahinduwe mu buryo bwa genoside binyuze mu nzira izwi nko guhindura gene.Guhindura Gene bikubiyemo guhindura ADN yibinyabuzima, akenshi hagamijwe kwiga genes runaka cyangwa gusobanukirwa inzira zimwe na zimwe z’ibinyabuzima.
Ukoresheje tekinoroji yo guhindura gene, birashoboka kugera kumurongo wimikorere ya selile, imirongo ya selile ikomanga, ihinduka ryimiterere, cyangwa gukomeretsa imirongo ya selile muri selile yihariye, ishobora noneho gukoreshwa mukwiga imikorere ya gene, ibimenyetso, uburyo bwindwara. , hamwe no guteza imbere ibiyobyabwenge, kimwe no kuranga selile zihariye.
Hariho impamvu nyinshi zituma imirongo ya selile ikosorwa ifite agaciro kubushakashatsi bwa siyansi.Ubwa mbere, batanga igikoresho cyo kwiga imikorere ya gen runaka.Muguhindura ingirabuzimafatizo ahantu hagenzuwe, abahanga barashobora kureba ingaruka zo guhindura gene kumyitwarire ya selile cyangwa mubindi binyabuzima.Ubu bumenyi bushobora kugira uruhare mu gusobanukirwa neza n'indwara zishingiye ku ngirabuzima fatizo, ndetse no kumenya intego zishobora kuvurwa.
Byongeye kandi, imirongo ya selile yahinduwe irashobora gukoreshwa mugutezimbere no gupima imiti mishya.Mugutangiza ihinduka ryimiterere yihariye, abahanga barashobora gukora imirongo yingirabuzimafatizo yigana imiterere yindwara, ibafasha gusuzuma akamaro kokuvura.Ibi bituma habaho uburyo bunoze kandi bunoze bwo guteza imbere ibiyobyabwenge.
Byongeye kandi, imirongo yahinduwe na selile ifite ubushobozi bwo gukoreshwa mubuvuzi bushya.Muguhindura ingirabuzimafatizo zishinzwe imyitwarire ya selile cyangwa itandukaniro, abahanga barashobora kongera ubushobozi bwo kuvura ingirabuzimafatizo cyangwa gukora selile zikwiranye no guhindurwa.
Ibyiza
1. Gene-yahinduwe na lokusi icyarimwe;
2. gutanga serivisi imwe iva mubishushanyo bya sgRNA hamwe na synthesis kugeza umurongo wa selile;
3. Uburambe bukomeye muri synthesis ya gene kugirango ifashe ibice byo guhindura gene kuva igishushanyo mbonera kugeza kurangiza kubaka.
Ubucuruzi Bukuru
- Gene Knock-out Line Cell
- Imirongo yo Gukora Gene
- Erekana imirongo ya mutant
- Gukomanga ku murongo w'akagari
Reba
[1] Zhang S, Shen J, Li D, Cheng Y. Ingamba zo gutanga Cas9ribonucleoprotein ya CRISPR / Cas9 yo guhindura genome.Theranostics.2021 Mutarama 1; 11 (2): 614-648.doi: 10.7150 / thno.47007.PMID: 33391496;PMCID: PMC7738854.